Impact of the extracts and fractions of Pennisetum purpureum on the locomotive ability of Drosophila melanogaster under oxidative stress condition

Yocy Yohana Izam, Kennedy Amagon and Sunday Yakubu Sabo

Advancement in Medicinal Plant Research
Published: April 5 2024
Volume 13, Issue 2
Pages 17-25

Abstract

Oxidative damage can adversely affect the climbing performance and acetylcholine levels of Drosophila melanogaster. This study evaluated the protective effects of Pennisetum purpureum on climbing performance and acetylcholine levels in fruit flies exposed to hydrogen peroxide (H₂O₂). In this study, fruit flies were exposed to various concentrations of extracts and fractions of P. purpureum. Climbing ability was assessed using the method described by Abolaji et al. (2020) and Sharma et al. (2022), while the acetylcholine assay was conducted following the protocols of Ream et al. (2003) and Makos et al. (2009). The results revealed a significant increase in climbing performance and acetylcholine levels in flies treated with various concentrations of extracts and fractions compared to the control (P > 0.05). The H₂O₂ treated control group exhibited the lowest values, which were significantly different from those of the treated groups. These findings suggest that the extracts and fractions of P. purpureum enhanced climbing ability and increased acetylcholine levels in D. melanogaster, indicating a protective effect against oxidative damage-induced locomotive impairment.

Keywords: Climbing performance, hydrogen peroxide, acetylcholine, P. purpureum, D. melanogaster, oxidative damage.

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