Production of nutraceutical compounds from Cannabis indica by different integrative treatments in nutritive solution

Victor M. Marín-Campos, Joel Pineda-Pineda, Mateo Vargas-Hernández, Gustavo Almaguer-Vargas, Rosa M. López-Romero and Cecilia García-Osorio

Advancement in Medicinal Plant Research
Published: April 12 2024
Volume 13, Issue 2
Pages 26-33

Abstract

Cannabis indica is a multipurpose species rich in phytochemical compounds with nutraceutical and medicinal properties. Mineral nutrition has been shown to be an important factor for the synthesis of these molecules. However, the influence of ion balances with signaling and stimulating molecules applied through the nutrient solution on the synthesis of metabolites has not been studied. Therefore, the purpose of this work was to delimit the impact of ion balances complemented with acetylsalicylic acid (ASA) and phenylalanine (Phe) on the development of the plant and on the production of nutraceutical compounds. Nine treatments were evaluated: 12 mmol_c L^-1; 15 mmol_c L^-1; 15 mmol_c L^-1 + 20 mg L^-1 ASA; 15 mmol_c L^-1 + 500 mg L^-1 Phe; 15 mmol_c L^-1 + 20 mg L^-1 ASA + 500 mg L^-1 Phe; 19 and 20 mmol_c L^-1; 19 and 20 mmol_c L^-1 + 20 mg L^-1 ASA; 19 and 20 mmol_c L^-1 + 500 mg L^-1 Phe; 19 and 20 mmol_c L^-1 + 20 mg L^-1 ASA + 500 mg L^-1 Phe in a randomized block design under controlled conditions. The commercial solution (12 mmol_c L^-1) performed better in terms of quercetin production (1878 mg 100 g^-1) in inflorescences, accompanied by an antagonism for Ca. The Hoagland solution (19 mmol_c L^-1) obtained better vegetative development with higher heights (9.9 - 10.8 cm) and SPAD indices (50.1 - 52.8). The combination of Hoagland and Steiner generated high levels of foliar potassium (7.74 to 8.86 %) due to cationic antagonisms, but produced lower concentration of quercetin. Supplementation of the nutrient solution promoted higher leaf biomass in treatments 3 and 8 with 8.3 g of dry matter, and improved calcium absorption in treatments 3 and 5.

Keywords: Hemp, phenylalanine, ionic balances, acetylsalicylic acid, flavonoids.

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