Effect of indole-3-acetic acid, indole-3-butyric acid and 1-naphthalene acetic acid on the stem cutting and vegetative growth of Lawsonia inermis L.

Mohammed Sala Uddin and Md. Abu Shale Musa

International Journal of Ecology and Ecosolution
Published: November 15 2024
Volume 10, Issue 1
Pages 10-17

Abstract

Conventional methods for the propagation of Lawsonia inermis L. encounter challenges such as limited seed viability, susceptibility to diseases and pests, and inconsistent seed propagation due to the conditions of their natural habitat, which hinder large-scale production. In vitro micropropagation also shows limitations for mass production, primarily due to low hardening rates and extended time requirements for generating the desired leaf biomass. Consequently, vegetative propagation through stem cuttings emerges as a practical approach to enhance productivity in leaf biomass by multiplying superior, healthy plants. The application of auxins significantly influences the rooting and shooting processes of cuttings. An experiment was conducted at the Botanic Garden, University of Chittagong, located in southeastern Bangladesh, to assess the effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) on the vegetative growth potential of L. inermis stem cuttings using the quick dip method and a completely randomized design. The results indicated notable variability in root and shoot development, which directly impacts the leaf biomass production of L. inermis cuttings. All three auxins, IAA, IBA, and NAA, demonstrated significant effects on the development of shoots and roots, except for control cuttings, which managed to develop sufficient roots without the application of hormones. The findings suggest that IBA-treated cuttings are more effective in producing higher leaf biomass compared to those treated with IAA and NAA, highlighting their potential importance in the herbal medicine sector and in economically developing nations such as Bangladesh. It is recommended to further explore the combined effects of IAA, IBA, and NAA, along with other plant growth regulators, on the rooting and shooting capabilities of L. inermis to maximize leaf biomass production.

Keywords: Lawsonia inermis, leaf biomass, IAA, IBA, NAA, vegetative growth potential.

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