The relationship between gene polymorphisms of coagulation factors II, V and XI and risk of recurrent pregnancy loss in Palestine

Abstract

This retrospective case-control study was carried out in order to investigate the association between Factor II (G20210A), Factor V (G1691A, H1299R, Y1702C) and Factor XI (rs3756008 A>T) gene polymorphisms and recurrent pregnancy loss (RPL) in a group of Palestinian women residing in Gaza strip. A total of 400 females, 200 RPL patients and 200 women without previous history of RPL, aged 20 to 35 years were included in the study. Factor V (G1691A) and Factor II (G20210A) polymorphisms were analyzed by allele-specific PCR whereas, Factor V (H1299R, Y1702C) and Factor XI (rs3756008 A>T) gene polymorphisms were screened by PCR-RFLP. No statistically significant difference existed between RPL cases and controls in terms of the allelic and genotypic distribution of Factor II (G20210A), Factor V (Y1702C) or Factor XI (rs3756008 A>T). On the other hand, the minor allele of the two Factor V gene polymorphisms (G1691A and H1299R) independently showed statistically significant association with RPL. Factor V G1691 and H1299R polymorphisms may increase the risk for RPL in Gaza strip women, and should be screened for in cases suspected to have inherited thrombophilia.

Keywords: Factor V, Factor II, Factor XI, gene polymorphism, recurrent pregnancy loss.