Molecular studies on E. coli isolate from milk of mastitic cattle with special reference to associated biochemical changes in Kaliobea Governorate

Abstract

This investigation was performed in Teaching Hospital and arm of Benha University in Moshtohor. The number of cows in this farm is 80 dairy cows. 40 of them had clinical signs of mastitis (inflammation in teats, pain in milking and milk decrease in amount and quality). The authors examined these cows to identify the cause of these signs. California Mastitis Test (CMT) was performed to determine positive milk samples in the mastitic targeted cows. 20 samples of early lactation stage cows were recovered from 40 CMT-positive milk samples. Biochemical and PCR tests were performed to isolates E. coli from positive milk samples (CMT) and determined three virulance genes, eae gene, SXT1 and SXT2. The significance of E. coli-induced mastitis and biochemical changes associated to it in cows, due to the presence of virulence genes and resistance to a wide range of 20 antimicrobials, is concluded. E. coli cause biochemical changes in mastitic cow as liver enzymes AST, GPT, TP. Oxidative enzymes as CAT, SOD, GST, LD and kidney function as urea and creatinine. E. coli has effect on inflammatory response in mastitic cow immunity system of by L6, TNF and CRP.

Keywords: Mastitis, serotyping characterization, PCR, biochemical alteration.