Influence of gamma irradiation on expressed heat shock proteins of Pantoea agglomerans, a yellow pigmented phytopathogen

Dominic Dussault, Khanh Dang Vu, Stephane Caillet, Canh Le Tien and Monique Lacroix

Microbiology Research International
Published: September 3 2015
Volume 3, Issue 3
Pages 51-55

Abstract

The aim of this study was to evaluate the effect of γ-radiations on the heat shock proteins (Hsps) content of strains of Enterobacteriacea and Pantoea agglomerans. Radiation treatments (1 and 3.5 kGy) were performed to highlight the radiotolerance. Total proteins were extracted after irradiation treatments and heat shock proteins (Hsp) of interest (GroEL, GroES and DnaK) were visualized and quantified using Western blot analysis. It was found that GroEL synthesis was increased significantly (P ≤ 0.05) for both strains while the DnaK seemed to be down regulated for the strain from ATCC 49174. Basal level of GroEL proteins was much higher in the strain isolated from carrots. The obtained results indicated the modification effects of γ-radiations on the Hsps at cellular level.

Keywords: Pantoea agglomerans, γ-irradiation, heat shock proteins, radiotolerance, western blot.

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